NASC Stock Detail Page
NASC ID: N569823
Name: SALK_069823
ABRC stock number: SALK_069823
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_069823.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G01695.1 and AT1G01700.1 (at chr 1 pos 257862 (W/257785-257862) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO359633. SALKseq_069823.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G11402.1 (at chr 3 pos 3575497 (C/3575497-3575536) on the TAIR10). The sequences' 1-40 bps mapped on the genome, 39-64 bps onto the T-DNA. Pools: P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO362988. SALKseq_069823.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G29103.1 (at chr 4 pos 14346091 (W/14346014-14346091) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools: P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO366328. SALKseq_069823.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G37000.1 (at chr 4 pos 17442563 (C/17442563-17442604) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO369674. SALKseq_069823.5 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G58660.1 (at chr 5 pos 23703978 (W/23703901-23703978) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 47, R 42, C 46 N 45. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO373034.
Donation Date: 2002-05-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_069823
Part of : SALK lines
Stock type: individual line
Material type: seed
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.